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Agriculture and forestry

space SCIENTIFIC JOURNALS OF VINNITSA NATIONAL AGRARIAN UNIVERSITY

Issue №: 4 (11)

Published: 1970.01.01
DOI: 10.37128/2707-5826-2018


Description:
Публікації ціого номера присвячено вивченню технологічних прийомів вирощування культур, а саме впливу попередників, регуляторів росту, біопрепаратів. Вивченню біологічного землеробства. Виробництва біодизелю із відновлювальних джерел енергії.

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MOLECULAR-BIOLOGICAL PROPERTIES OF ENTOMOPATHOGENIC STRAIN BACILLUS THURINGIENSIS 87/3

DOI: XXXXX.XXXXXXXX
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Boyko Mariya Victorivna – post-graduate student of the Department of Ecobiotechnology and Biodiversity, National University of Life and Environmental Sciences of Ukraine (03041, Kyiv, Heroes of Defense Str., 13 e-mail: maryaulina@gmail.com).

Patyka Tatyana Ivanivna – Doctor of Agricultural Sciences, Director of the Institute of Phytomedicine, Biotechnology and Ecology, Senior Researcher of the Department of Phytopathology, National University of Life and Environmental Sciences of Ukraine (03041, Kyiv, Heroes of Defense Str., 13 e-mail: patykatatyana@gmail.com).

Patyka Nikolay Vladimirovich – Doctor of Agricultural Sciences, Corresponding Member of the National Academy of Sciences of Ukraine, Head of the Department of Ecobiotechnology and Biodiversity, National University of Bioresources and Nature Management of Ukraine (03041, Kiev, Heroes of Defense, 13, e-mail: ecobio.chair@gmail. com).

Annotation

The comparative analysis of the main physiological and biochemical properties of entomopathogenic bacteria Bacillus thuringiensis (Bt) 87/3 strain with similar properties of the Bt 800 reference strain has been carried out. It has been observed that in terms of cultural, morphological, physiological and biochemical properties, in particular the breakdown of starch and esculin, the formation of catalase, AMC, the liquefaction of gelatin, strain belongs to the B.thuringiensis group. The molecular-biological analysis of the Bt 87/3 strain was carried out, based on the study of the 16S rRNA nucleotide sequences genes polymorphism. Based on the analysis of the 16S rRNA nucleotide sequence, a dendrogram of phylogenetic connections between different representatives of the bacteria genus Bacillus was constructed. The 16S rRNA strain sequence was registered and entered into the GenBank database (MH719010). The conducted research creates a basis for the further genotype culture studying that are perspective to use in biotechnology.

Keywords: Bacillus thuringiensis, cultural-morphological characteristics, physiological-biochemical properties, intraspecific heterogeneity, molecular biological analysis, identification.

List of references

1.Kandibyn N. V., Patika T. Y., Ermolova V. P., Patika V. F. (2009)Mykrobyokontrol chyslennosty nasekomikh y eho domynanta Bacillus thuringiensis. [Microbiogical of insects and its dominant Bacillus thuringiensis] Sankt-Peterburh: Nauchnoe yzdanye «Ynnovatsyonnii tsentr zashchyti rastenyi». [in Ukrainian].

2.Bravo A., Gomez I., Porta H. et al. (2013) Evolution of Bacillus thuringiensisCry toxins insecticidal activity. Microbial Biotechnol. Vols. 6, 17-26. [in Ukrainian].

3.Yudina T.G. (2006) Antimikrobnaya aktivnost i ekologicheskaya rolbelkovyih vklyucheniy bakteriy – predstaviteley rodov Bacillus [Antimicrobial activity and ecological role of protein inclusions of bacteria - representatives of Bacillus genera]. Moskva. [in Russian].

4.Prakash O., Verma M., Sharma P. (2007). Polyphasic approach of bacterialclassification – an overview of recent advances. Indian J. Microbiol. Vols. 47. 2, 98-100. (In India).

5.Smirnov V. V., Reznik S. R., Sorokulova I. B. (1983) Metodicheskierekomendatsii po vyideleniyu i identifikatsii bakteriy roda Bacillus iz organizma cheloveka i zhivotnyih. Kiev [Guidelines for the isolation and identification of bacteria of the genus Bacillus from the human body and animals]. Kyiv: Naukova dumka. [in Ukrainian].

6.Smirnoff W. A. A (1962) straining mеthod for differentiating spores, crystalsand cells of Bacillus thuringiensis. Insect. Pathol. Vols 3, 384-386. [in United States].

7.Wilson K. (2001) Preparation of genomic DNA from bacteria. Currentprotocol in Mol. Biol. 2.4.1-2.4.5. [in United States].

8.Patyka M.V., Patyka T.I., Grygoryuk I.P. (2012) Molekulyarno-genetychnyjanaliz polimorfizmu metagenomnyx nukleotydnyx poslidovnostej entomopatogennyx bakterij Bacillus thuringiensis i prokariotnogo kompleksu gruntiv. Dopovidi Nacionalnoyi Akademiyi Nauk Ukrayiny. Texnichni nauky: naukovo - teoretychnyj zhurnal Prezydiyi NANU – Scientific and Theoretical Journal of the Presidium of the National Academy of Sciences of Ukraine. 1, 164-170. [in Ukrainian].

9.Tamura K., Stecher G., Peterson D., et. al. (2013) MEGA6: MolecularEvolutionary Genetics Analysis version 6.0. Mol. Biol. Evol. Vols. 30. 2725-2729. [in United States].

10.Keshavarzi M. (2008) Isolation, Identification and differentiation of localBacillus thuringiensis strains. J. Agric. Sci. Technol. Vols. 10. 2. 43-49 [in Iran].

11.Goto K., Omura T., Hara Y., Sadaie Y. (2000) Application of the partial 16SrDNA sequence as an index for rapid identification of species in the genus Bacillus. J. Gen. Appl. Microbiol. Vols. 46. 11-18. [in Japan].

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About journal

Topics of the journal:

Topics of the Journal:

Program goals (basic principles): questions of technology and efficiency of crop production, scientific and industrial aspects of agronomic specialties, ecology and forestry.

Articles on such scientific problems are accepted for printing:

Plant production, current state and prospects for development.

Agrochemistry and modern aspects of fertilizers and biologically active substances application.

Feed production, current state and prospects for development.

Selection, seed production, seed science and varieties.

Protection of plants.

Soil managment and soil quality improvement.

Forestry and gardening.

Vegetables and mushrooms, current status and development trends.

Ecology and environmental protection.

Theory and history of agricultural sciences and forestry.

Modern directions of development of arable land and forestry.

Innovative and investment activities in agriculture and forestry.

Directions and efficiency of crop production

Bioenergy resources of crop production and forestry.

Production of renewable energy from biological resources.

Organic farming, the main area of development and production of organic products.

Agriculture and modern crop rotation and soil cultivation.

Horticulture and viticulture.

Processing and storage of crop products.

Beekeeping, current status and development trends.

Contemporary biological research in crop production and forestry.

Progressive technologies of the agricultural complex.

Psychological and pedagogical problems of agrarian education.

The young scientist opinion.

 

Key information:
ISSN (print): 2707-5826
DOI: 10.37128/2707-5826
Key information:
ISSN (print): 2707-5826
DOI: 10.37128/2707-5826
Scientific journal "AGRICULTURE AND FORESTRY" was founded  in 1995;  it was entitled as  Bulletin of Vinnytsya State Agricultural Institute.
In 2010-2014 was enritled as Collection of scientific works of Vinnytsia National Agrarian University.
It has been named as Agriculture and Forestry since 2015.
 
Certificate of mass media state registration No. 21363-11163 Р of June 09, 2015
1. Founder (co-founder): Vinnytsia National Agrarian University.
2. Type of publication: journal.
3. Publication status: domestic.
4. Title(s) of publication: Agriculture and Forestry.
5. Language(s) of the publication: Ukrainian and English.
6. Spread: nationwide.
7. Categories of readers: students, postgraduates, scientists, employees of agroindustrial complex, state bodies of management.
8. Program goals (basic principles): questions of technology and efficiency of crop cultivation, scientific and industrial aspects of agronomic specialties, ecology and forestry.
9. Estimated periodicity of issue, volume (in conventional printed sheets) and format of publication: twice a half year, 7.5-15 conventional printed sheets, format: A4.
10. Legal address of the founder: 21008, Vinnytsia, Soniachna Street, 3; telephones: (0432) 56-03-13; 35-70-84, fax: (0432) 43-80-25; e-mail:
11. Location of the editorial office: 21008, Vinnytsia, Soniachna Street, 3.
12. Type of publication by purpose: agriculture and forestry, scientific production.
History of journal:

Journal History:

Scientific journal "AGRICULTURE AND FORESTRY" was founded  in 1995;  it was entitled as  Bulletin of Vinnytsya State Agricultural Institute.

In 2010-2014 was enritled as Collection of scientific works of Vinnytsia National Agrarian University.

It has been named as Agriculture and Forestry since 2015.

Listed in the updated list of scientific professional editions of Ukraine on agricultural sciences under the name Agriculture and Forestry (Order of the Ministry of Education and Science of Ukraine of May 16, 2016; No. 515).

Since 2015, "Agriculture and Forestry".  (archive of previous issues for 2015-2019) at http://forestry.vsau.edu.ua/.

The journal “Agriculture and Forestry (ISSN: 2707-5826)” passed the evaluation process of Index Copernicus positively and is indexed in the database of ICI Journals Master List   ICV for 2018, 2019, 2020, 2021, 2022 is presented in the full list of indexed journals in the Master List International List 

https://journals.indexcopernicus.com/search/details?id=65401

According to the order of the Ministry of Education and Science of Ukraine dated 17.03.2020 № 409. The journal Agriculture and Forestry is included in the List of scientific professional publications of Ukraine Category "B" in agricultural sciences (specialties) - 101, 201, 202, 203, 205, 206.